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Category
Primary Antibodies
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Long description
This antibody binds to JNK2 protein, a protein with Mr=46 or 54 kDa. JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.
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Antibody come from
KLH conjugated peptide corresponding to amino acid residues 519-536 of human c-Src.
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Other description
Provided as solution in phosphate buffered saline, pH 7.2, with 1% BSA and 0.1% sodium azide. Protein A/G Chromatography
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Clone
341P16
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Antigen antibody binding interaction
Mouse anti JNK2 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA, Western Blot
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Antibody s suited for
This antibody is suitable for use in Western blotting, immunocytochemistry and immunohistochemistry. For Western blotting, the recommended concentration is 1 µg/mL. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Kawakatsu, H., et al. (1996) A new monoclonal antibody which selectively recognizes the active form of Src tyrosine kinase. J. Biol. Chem. 271(10):5680-5685._x000B_2. Sakai, T., et al. (1998) An epitope localized in c-Src negative regulatory domain is a potential marker in early stage of colonic neoplasms. Lab. Invest. 78(2):219-225._x000B_3. Shanmugam, M., et al. (1998) Association of PKC delta and active Src in PMA-treated MCF-7 human breast cancer cells. Oncogene 16(13):1649-1654._x000B_4. Kovacic, B., et al. (1998) c-Src activation plays a role in endothelin-dependent hypertrophy of the cardiac myocyte. J. Biol. Chem. 273(52):35185-35193._x000B_5. Luttrell, L.M., et al. (1999) Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Science 283(5402):655-661._x000B_6. Wu, Y., et al. (2000) Differential activation and redistribution of c-Src and Fyn in platelets, assessed by MoAb specific for C-terminal tyrosine-dephosphorylated c-Src and Fyn. Biochem. Biophys. Acta 1497(1):27-36._x000B_7. Yamada, T., et al. (2000) Scraped-wounding causes activation and association of c-Src tyrosine kinase with microtubules in cultured keratinocytes. Cell. Struct. Funct. 25(6):351-359._x000B_8. Yamamoto, Y., et al. (2002) Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells. Mol. Hum. Reprod. 8(12):1117-1124._x000B_9. Ghalayini, A.J., et al. (2002) Light-dependent association of Src with photoreceptor rod outer segment membrane proteins in vivo. J. Biol. Chem. 277(2):1469-1476._x000B_10. Zhao, Y.L., et al. (2003) Active Src expression is induced after rat peripheral nerve injury. Glia. 42(2):184-193._x000B_11. Takikita-Suzuki, M., et al. (2003) Activation of Src kinase in platelet-derived growth factor-B-dependent tubular regeneration after acute ischemic renal injury. Am. J. Pathol. 163(1):277-286.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
