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Category
Primary Antibodies
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Long description
JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress._x000B__x000B_This antibody binds to both JNK1 and JNK2 proteins of Mr=46 and 54 kDa_x000B_
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Antibody come from
Recombinant human c-Src expressed in E. coli.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1% BSA and 0.08% sodium azide. Protein A/G Chromatography
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Clone
279Q38
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Antigen antibody binding interaction
Mouse anti JNK1/2 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western blotting
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Antibody s suited for
Antibody can be used for Western blotting (0.5-1 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Martin, G.S. (2004) The road to Src. Oncogene 23(48):7910-7917._x000B__x000B_2. Haas, A., et al. (2002) Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J. Biol. Chem. 277(21):18694-18702._x000B__x000B_3. Bourguignon, L.Y.W., et al. (2001) CD44 interaction with c-Src kinase promotes cortactin-mediated cytoskeleton function and hyaluronic acid-dependent ovarian tumor cell migration. J. Biol. Chem. 276(10):7327-7336._x000B__x000B_4. Ushio-Fukai, M., et al. (2001) Cholesterol detection inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells - Role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling. J. Biol. Chem. 276(51):48269-48275._x000B__x000B_5. Vara, J.A.F., et al. (2001) Src family kinases are required for prolactin induction of cell proliferation. Mol. Biol. Cell 12(7):2171-2183._x000B__x000B_6. Ma, Y.-C., et al. (2000) Src tyrosine kinase is a novel direct effector of G proteins. Cell 102:635-646._x000B__x000B_7. Suter, D.M. and P. Forscher (2001) Transmission of growth cone traction force through apCAM-cytoskeletal linkages is regulated by Src family tyrosine kinase activity. J. Cell Biol. 155(3):427-438.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
