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Category
Primary Antibodies
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Long description
Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
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Antibody come from
D33 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified desmin from a leiomyosarcoma.
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Other description
Each vial contains 100ul 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide
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Clone
D33
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Antigen antibody binding interaction
Mouse anti Desmin Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Hamster, Rat, Chicken
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The antibody D33 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma in human, hamster, rat and chicken. The antibody does not cross react with vimentin or any of the other intermediate filament proteins.
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Research interest
Cell Biology
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Application
Immunocytochemistry, Immunohistochemistry (Frozen, Paraffin), Western Blotting
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Antibody s suited for
The D33 antibody is suitable for the detection of desmin by Western blotting and detects a band of 53kDa in muscle tissue extracts. The antibody is also suitable for the detection of desmin by immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. The antibody works on paraffin-embedded material without pretreament with proteolytic enzymes, but the section should be pre-treated using heat mediated antigen retrieval with Tris-buffer containing 1 mM EDTA, pH 9,0 for 20 mins. Optimal antibody dilutions for the different applications should be determined by titration.
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Storage
Store at 4oC, or in small aliquots at -20oC.
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Relevant references
1. Van Muijen GN, Ruiter DJ, Warnaar SO. (1987) Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest. 57:359-69 2. Pollock L, Rampling D, Greenwald SE, Malone M. (1995) Desmin expression in rhabdomyosarcoma: influence of the desmin clone and immunohistochemical method. J Clin Pathol. 48:535-8. 3. Fetsch JF, Miettinen M, Laskin WB, Michal M, Enzinger FM. (2000) A clinicopathologic study of 45 pediatric soft tissue tumors with an admixture of adipose tissue and fibroblastic elements, and a proposal for classification as lipofibromatosis. Am J Surg Pathol. 24:1491-500. 4. Chang TK, Li CY, Smithson WA. (1989) Immunocytochemical study of small round cell tumors in routinely processed specimens. Arch Pathol Lab Med.113:1343-8. 5. Pohar-Marinsek Z, Srebotnik-Kirbis I. (2000) Desmin detection in FNAB samples of rhabdomyosarcoma: an immunocytochemical study. Cytopathology 11:171-8.
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Protein number
P17661
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Gene
Desmins or DES proteins detect by anti-desmin antibodies and DES ELISA kits. DSM1 and DSM2 play an important role in Desminopathy. Desmin regulated Myofibrilate Myopathy is a protein filament mutation of muscle proteins.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
