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Category
Primary Antibodies
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Long description
CD8-PECy5 is a monoclonal antibody (MAb) labelled with the R-phycoerythrin-cyanine 5 tandem (PECy5) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD8 antigen by flow cytometry._x000B__x000B__x000B_Flow Cytometry is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cells in liquid suspension that have been incubated with fluorescently-labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression._x000B__x000B_Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity._x000B__x000B_The CYT-8C2 MAb recognizes the CD8 antigen present in suppressor/cytotoxic T cells (CD3+CD8+), and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc (1,2).
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Antibody come from
CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells.
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Other description
Provided as solution in phosphate buffered saline with 0.1% sodium azide. Affinity chromatography
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Clone
17D8
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Antigen antibody binding interaction
Mouse anti CD8 antibody conjugated to Cy5-PE Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
WARNINGS AND RECOMMENDATIONS_x000B_1.For in vitro diagnostic use. _x000B_2.This product is supplied ready to use. If it is altered by dilution or addition of other components, it will be invalidated for in vitro_x000B_diagnostic use. _x000B_3.The reagent is stable until the expiration date shown on the label if it is properly stored. Do not use after the expiration date shown_x000B_on the label. If the reagents are stored in conditions different from those recommended, such conditions must be validated by the_x000B_user. _x000B_4.Alteration in the appearance of the reagent, such as the precipitation or discoloration indicates instability or deterioration. In such_x000B_cases, the reagent should not be used. _x000B_5.It contains 0.1% sodium azide (CAS-Nr. 26628-22-8) as a preservative, but even so care should be taken to avoid microbial_x000B_contamination of reagent or incorrect results may occur. _x000B_ ¥Sodium azide (NaN3) is harmful if swallowed (R22), if swallowed, seek medical advice immediately and show this_x000B_container or label (S46). _x000B_ ¥Wear suitable protecting clothing (S36). _x000B_ ¥Contact with acids liberates very toxic gas (R32). _x000B_ ¥Azide compounds should be flushed with large volumes of water during disposal to avoid deposits in metal drains where_x000B_explosive conditions may develop._x000B_6.All patient specimens and materials with which they come into contact are considered biohazards and should be handled as if capable of transmitting infection (5), and disposed according to the legal precautions established for this type of product. Also recommended is handling of the product with appropriate protective gloves and clothing, and its use by personnel sufficiently qualified for the procedures described. Avoid contact of samples with skin and mucous membranes. After contact with skin, wash immediately with plenty of water._x000B_7.Use of the reagent with incubation times or temperatures different from those recommended may cause erroneous results. Any such changes must be validated by the user._x000B__x000B_WARRANTY_x000B_This product is warranted only to conform to the quantity and contents stated on the label. There are no warranties that extend beyond the description on the label of the product. CytognosÕs sole liability is limited to either replacement of the product or refund of the purchase price.
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Storage
4ºC
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Relevant references
1.Orfao A, Gonz‡lez de Buitrago JM La citometr’a de flujo en el laboratorio cl’nico. Sociedad espa–ola de bioqu’mica cl’nica y patolog’a Molecular 1995._x000B_2. Stetler-Stevenson M. Flow cytometry analysis of lymphomas and lymphoproliferative disorders. Semin Hematol 2001 Apr;38(2):111-23._x000B_3.MenŽndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)_x000B_4.GratamaJW, MenŽndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)_x000B_5.Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_6.Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_7.Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B_8.Lima M et al. TCR +/CD4+ Large Granular Lymphocytosis. A new Clonal T-Cell Lymphoprolipherative Disorder. American Journal of Pathology, 163 (2):763-771 (2003)_x000B_9.Lima M. et al. Utility of flow cytometry immunophenotyping and DNA ploidy studies for diagnosis and characterization of blood involvement in CD4+ SŽzary s syndrome. Haematologyca 88(8): 874-887(2003)._x000B_10. Gorczyza W. et al. An approach to diagnosis of T-cell lymphoprolipherative disorders by flow cytometry. Cytometry (Clinical cytometry) 50:177-190 (2002)_x000B_11.Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991) 12.Prince HK et al. Influence of racial background on the distribution of T-cell subsets and Leu-11 positive lymphocytes in healthy_x000B_blood donors. Diagn Immunol. 3: 33-39 (1985) 13.Kotylo PK et al. Reference ranges for lymphocyte subsets in pediatric patients. Am J Clin Pathol 100:111-5 (1993)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
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Conjugation
Cy5.5
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
