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Category
Primary Antibodies
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Long description
Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.
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Antibody come from
CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells.
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
M28623
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Antigen antibody binding interaction
Mouse anti CD5 antibody conjugated to APC Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
PBMC : Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
1. Anti-DNA Antibody Production by CD5+ and CD5- B Cells of Patients with Systemic Lupus Erythematosus. Suzuki N., Sakane T., Engleman EG, J. Clin. Invest. 1990 Ja;85(1):238-47. _x000B__x000B_2. Characteristics of CD11c+ CD5+ Chronic B cell Leukemias and the Identification of Novel Peripheral Blood B cell Subsets with Chronic Lymphoid Leukemia Immunophenotypes. Wormsley SB, Baird SM, Gadol N., Rai KR, Sobol RE, Blood 1990 July. 76(1):12330. _x000B__x000B_3. Stimulation of CD5 Enhances Signal Transduction by the T cell Antigen Receptor. Imboden JB, June CH, McCutcheon MA, Ledbetter JA, J. Clin. Invest. 1990 Ja;85(1):130-4. _x000B__x000B_4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5B Lymphocytes. Nawata Y., Stall Am, Herzenberg LA, Eugui Em, Allison AC, Int. Immunol. 1990;2(7):603-14. _x000B__x000B_5. Evidence for Differential Responsiveness of Human CD5+ and CD5- B cell Subsets to T cell Independent Mitogens. Zupo S., Dono M., Azzoni L., Chiorazzi N., Ferrarini M., Eur. J. Immunol. 1991 Fe. 21(2):351-9.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD5, APC
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Short name
Mouse anti CD5 antibody conjugated APC
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Technique
Antibody, Mouse, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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Host
mouse
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Isotype
IgG2a
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Label
APC
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to CD5 molecule (antibody to-) coupled to adenomatous polyposis coli
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Alternative technique
antibodies, murine
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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