Mouse anti CD38 antibody conjugated to FITC
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Catalog number0382
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PricePlease ask
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Size100 Tests
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CategoryPrimary Antibodies
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Long descriptionCD38 antigen is an integral membrane glycoprotein, M.W. 45 kD. The CD38 antigen is present on approximately all pre-B lymphocytes, plasma cells and thymocytes. CD38 antigen is also expressed on activated T Lymphocytes, NK Cells, monocytes, myeloblasts and erythroblasts. CD38 is co-expressed on CD34+ cells,but is not expressed on pluripotent stem cells. CD38 antigen is present on T and B lymphoblastic leukemia (ALL), Burkitts' lymphoma, multiple myeloma and acute myeloid leukemia (AML).
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Antibody come fromn/a
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Other descriptionProvided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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CloneTME-2
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Antigen antibody binding interactionMouse anti CD38 antibody conjugated to FITC Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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Research interestCD Marker
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ApplicationFlow Cytometry
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Antibody s suited forPBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage4ºC
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Relevant references1) CD38 Molecule: Structural and Biochemical Analysis on Human T Lymphocytes, Thymocytes and Plasma Cells. J. Immunol, 1990 Aug; 145(3):878-84. Alessio, M. Roggero, S.; Funaro, A. ; De Monte L.B.,: Peruzzi, L.; Geuna, M.; Malavasi,F. _x000B__x000B_2) Differences Amoung Mononuclear Cell Subpopulations in HIV Seropositive or Seronegative Homosexual and Heterosexual men as Determined by Four Color Flow Cytometry. J. Acquir Immume Defic Syndr., 1990;3(8):747-56. Schnizlein-Bick, C.T.; Magier, M.R.; Jones, R.B.; Fife, K.H.; Katz, B., P.; Walker, E.B. _x000B__x000B_3) Quantitative Comparison of Myeloid Antigens on Five Lineages of Mature Peripheral Blood Cells. J. Leukoc Bio. 1990, Au;48(2):13848. Terstappen, L.W.; Hollander, Z.; Meiners, H.; Loken, M.R. _x000B__x000B_4) B-cell Surface Phenotypes of Proliferating Myeloma Cells: Target Antigens for Immunotherapy. Am. J. Hematol. 1990, Fe;33(2):1019, Chan, C.S; Wormsley, S.B.; Pierce, L. E.; Peter, J.B.; Schechter, G.P. _x000B__x000B_5) Interrelationships Between Serologic Markers of Immune Activation and T Lymphocyte Subsets in HIV Infection. J. Acquir Immune Defic Syndr. 1990;3(5):525-30. Prince, H.E.; Kleinman, S. Czaplicki, C.; John, J.; Williams, A.E. _x000B__x000B_6) Sequential Gererations of Hematopoietic Colonies Derived from Single Nonlineage-committed CD34+ CD38- Progenitor Cells. Blood 1991, Mar 1;77(6):1218-27. Terstappen, L.W.; huang, S.; Safford, M. Lansdorp, P.M.; Loken, M.R. _x000B__x000B_7) Involvement of Multilineage CD38 Molecule in a Unique Pathway of Cell Activation and Proliferation. J. Immunol, 1990 Oct 1;145(8):2390-6Funaro, A.; Spagnoli, G.C.; Ausiello, C.M.; Alessio, M.; Roggero, S.; Delia, D.; Zaccolo, M.; Malavasi, F. _x000B__x000B_8) Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Nat.349(6309): 5335, 1991. Lusso, P.; De Maria, A.; Malnati, M.; Lori, F.; DeRocco, S.E.;Baseler, M.; Gallo, R.C. _x000B__x000B_9) Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Clin. Res. 29, 365a, 1981. Engleman, E. G.; Benike, C. J.; Evans, R.L _x000B__x000B_10) CD4 and CD8 Molecules Can Physically Associate with the Same T-cell Receptor. Proc. Nat. Acad. Sci. 1989, 86:10044.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD38
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Short nameMouse anti CD38 antibody conjugated FITC
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TechniqueAntibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
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Hostmouse
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IsotypeIgG1
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to CD38 molecule (antibody to-) coupled to fluorecein
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD38 molecule, ADPRC 1 and T10, CD38 and IDBG-10668 and ENSG00000004468 and 952, hydrolase activity, nuclei, Cd38 and IDBG-161475 and ENSMUSG00000029084 and 12494, CD38 and IDBG-643982 and ENSBTAG00000013569 and 327677
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Gene info
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Identity
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Gene
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Long gene nameCD38 molecule
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Synonyms gene name
- CD38 antigen (p45)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data