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Category
Primary Antibodies
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Long description
The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is expressed on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M. W. surface antigen. CD62L is the equivalent of the mouse MEL-14 with a molecular weight of 80 KDa. CD62L is on 60-75% of peripheral blood lymphocytes, Approximately 70-80% of T cells, 10% of thymocytes and most B cells. CD62L reacts with monocytes, granulocytes and a subset of NK cells. The selectins represent a family of adhesion molecules, expressed by leukocytes and endothelial cells that are involved in the regulation of leukocyte traffic. Human leukocyte L selectin is also called LAM-1, LECAM-1, or gp90MEL-14. This antibody to L selectin blocks greater than 95% of lymphocyte adhesion to peripheral lymph node high endothelial venules.
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Antibody come from
CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD62L=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with perherial blood T lymphocytes.
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
not specified
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Antigen antibody binding interaction
Mouse anti CD3 antibody conjugated to FITC - CD62L antibody conjugated to PE Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼ C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
1. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259. _x000B__x000B_2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293. _x000B__x000B_3. Signal transduction via CD4,CD8 and CD28 in mature and immature thymocytes. Implications for thymic selection. Turka LA, Linsley PS, Paine R 3d, Schieven Gl, Thompson GB, Ledbetter JA, J. Immunol. 1991 Mar :146(5): 1428-36. _x000B__x000B_4. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9. _x000B__x000B_5. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21. _x000B__x000B_6. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23. _x000B__x000B_7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. _x000B__x000B_8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. _x000B__x000B_9. Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. J Cell Biol 1992 Jul;118(2):445-56. Moore KL; Stults NL; Diaz S; Smith DF; Cummings RD; Varki A; McEver RP _x000B__x000B_10. Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway. Mol Biol Cell 1992 Mar;3(3):309-21. Disdier M; Morrissey JH; Fugate RD; Bainton DF; McEver RP _x000B__x000B_11. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 1991 May 31;65(5):859-73. Lawrence MB; Springer TA _x000B__x000B_12. Biochemical and immunohistochemical characteristics of CD62 and CD63 monoclonal antibodies. Expression of GMP-140 and LIMP-CD63 (CD63 antigen) in human lymphoid tissues. Virchows Arch B Cell Pathol 1991;61(4):269-77. Metzelaar MJ; Schuurman HJ; Heijnen HF; Sixma JJ; Nieuwenhuis HK _x000B__x000B_13. CD62/P-selection recognition of myeloid and tumor cell sulfatides. Cell 1991 Oct 4;67(1):35-44. Aruffo A; Kolanus W; Walz G; Fredman P; Seed B _x000B__x000B_14. Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1. J Biol Chem 1991 Feb 5;266(4):2466-73. Collins T; Williams A; Johnston GI; Kim J; Eddy R; Shows T; Gimbrone MA Jr; Bevilacqua MP
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
