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Category
Primary Antibodies
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Long description
CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
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Antibody come from
n/a
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
OFYS
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Antigen antibody binding interaction
Mouse anti CD11a Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
-20ºC
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Relevant references
1. Protein kinase C-mediated regulation of the expression of CD14 and CD11/CD18 in U937 cells. Pedrinaci S; Ruiz-Cabello F; Gomez O; Collado A; Garr Int J Cancer 1990 Feb 1;45(2):294-8 _x000B__x000B_2. Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils. Hartnell A; Moqbel R; Walsh GM; Bradley B; Kay AB Immunology 1990 Fe;69(2):264-70 _x000B__x000B_3. Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Arnaout MA Immunol Rev 1990 Ap;114 p145-80 _x000B__x000B_4. Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells. Scand J Immunol 1990 Au;32(2):137-47 _x000B__x000B_5. Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. van Dijken PJ; Ghayur T; Mauch P; Down J; Burakoff SJ; Ferrara JL Transplantation 1990 Ma;49(5):882-6 _x000B__x000B_6. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Patarroyo M; Prieto J; Rincon J; Timonen T; Lundberg C; Lindbom L; Asjo B; Gahmberg CG Immunol Rev 1990 Ap;114 p67-108 _x000B__x000B_7. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins Anderson DC and Springer TA, Ann Rev Med 1987;38:175
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
