-
Category
Primary Antibodies
-
Long description
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is down-regulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.
-
Antibody come from
Synthetic peptide derived from the C-terminal human Lass1 protein.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
-
Clone
4A3
-
Antigen antibody binding interaction
Mouse anti Active Matrix Metalloproteinase 9 (MMP9) Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot, Immunohistochemistry(paraffin sections)
-
Antibody s suited for
Antibody can be used for Western blotting (1-5 µg/ml starting dilution). Other uses not yet tested. Only reacts with human Lass1 protein. Does not cross react with other species. Optimal concentration should be evaluated by serial dilutions.
-
Storage
-20ºC
-
Relevant references
[1] Lee S.-J.; Expression of growth/differentiation factor 1 in the nervous system: conservation of a bicistronic structure.; Proc. Natl. Acad. Sci. U.S.A. 88:4250-4254(1991)._x000B__x000B_[2] Jiang J.C., Kirchman P.A., Zagulski M., Hunt J., Jazwinski S.M.; Homologs of the yeast longevity gene LAG1 in Caenorhabditis elegans and human.; Genome Res. 8:1259-1272(1998)._x000B__x000B_[3] Grimwood J., et al.; The DNA sequence and biology of human chromosome 19.;_x000B_Nature 428:529-535(2004)._x000B__x000B_[4] The MGC Project Team; The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).; Genome Res. 14:2121-2127(2004).
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
MMP9
-
Short name
Mouse anti Active Matrix Metalloproteinase 9 (MMP9)
-
Technique
Mouse, anti, antibody to, mouses
-
Host
mouse
-
Isotype
IgG1
-
Label
unlabeled
-
Species
Mouse, Mouses
-
Alternative name
Mouse antibody to functionnal Matrix Metalloproteinase 9 (matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase))
-
Alternative technique
murine, antibodies
-
Alternative to gene target
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), CLG4B and GELB and MANDP2 and MMP-9, MMP9 and IDBG-78722 and ENSG00000100985 and 4318, identical protein binding, Extracellular, Mmp9 and IDBG-212451 and ENSMUSG00000017737 and 17395, MMP9 and IDBG-642990 and ENSBTAG00000020676 and 282871
-