GPR4 Blocking peptide

• Catalog number
  X1646B
• Price
  Please ask
• Size
  50 μg

• Category
  Proteins & Peptides
• Long description
  Sphingosylphosphorylcholine (SPC) and lysophosphatidylcholine (LPC) are bioactive lipid molecules involved in numerous biologicals processes. GPR4 shares sequence homology with OGR1 (51%), the highest of all GPCRÕs. GPR4 has been identified as another high affinity receptor for SPC and low affinity receptor for LPC. SPC and LPC stimulate kinase activation and DNA synthesis stimulated, both of which are pertussis toxin-sensitive, suggesting Gi-heterotrimeric G protein involvement. The GPR4 subfamily of GPCRÕs consists of four receptors that share significant sequence homology; OGR1, TDAG8 and G2A. G2A has been shown to be a potent transforming oncogene. GPR4 also malignantly transforms NIH3T3 cells and TDAG8 malignantly transforms the normal mammary epithelial cell line NMuMG. Overexpression of GPR4 or TDGA8 in HEK293 cells leads to transcriptional activation independent of exogenously added ligand. TDAG8 and GPR4 are also overexpressed in a range of human cancer tissues suggestive of a driving role in maintaining tumor formation.
• Antibody come from
  Recombinant enyzme produced in E. coli
• Other description
  Provided in 25 mM Tris-HCl, pH 8.0, 67 mM NaCl, 50% glycerol, 1.4 mM KCl, 3 mM DTT, 10 mM glutathione.
• Clone
  not specified
• Antigen antibody binding interaction
  GPR4 Blocking peptide
• Antibody is raised in
  see techfile
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Antibody function blocking
• Antibody s suited for
  1070 pmole/min/µg of enzyme; Detemined using pNPP; Reaction conditions: 1 mM pNPP, 15 min incubation at room temperature, 2 ng/µl enzyme.
• Storage
  -20ºC
• Relevant references
  1: Huynh H, Bottini N, Williams S, Cherepanov V, Musumeci L, Saito K, Bruckner S, Vachon E, Wang X, Kruger J, Chow CW, Pellecchia M, Monosov E, Greer PA, Trimble W, Downey GP, Mustelin T._x000B_Control of vesicle fusion by a tyrosine phosphatase. Nat Cell Biol. 2004 Sep;6(9):831-9. Epub 2004 Aug 22. _x000B__x000B_2:Huynh H, Wang X, Li W, Bottini N, Williams S, Nika K, Ishihara H, Godzik A, Mustelin T._x000B_Homotypic secretory vesicle fusion induced by the protein tyrosine phosphatase MEG2 depends on polyphosphoinositides in T cells. J Immunol. 2003 Dec 15;171(12):6661-71. _x000B__x000B_3: Xu MJ, Sui X, Zhao R, Dai C, Krantz SB, Zhao ZJ._x000B_PTP-MEG2 is activated in polycythemia vera erythroid progenitor cells and is required for growth and expansion of erythroid cells. Blood. 2003 Dec 15;102(13):4354-60. Epub 2003 Aug 14. _x000B__x000B_4: Zhao R, Fu X, Li Q, Krantz SB, Zhao ZJ._x000B_Specific interaction of protein tyrosine phosphatase-MEG2 with phosphatidylserine. J Biol Chem. 2003 Jun 20;278(25):22609-14. Epub 2003 Apr 17. _x000B__x000B_5: Qi Y, Zhao R, Cao H, Sui X, Krantz SB, Zhao ZJ._x000B_Purification and characterization of protein tyrosine phosphatase PTP-MEG2._x000B_J Cell Biochem. 2002;86(1):79-89. _x000B__x000B_6: Wang X, Huynh H, Gjorloff-Wingren A, Monosov E, Stridsberg M, Fukuda M, Mustelin T._x000B_Enlargement of secretory vesicles by protein tyrosine phosphatase PTP-MEG2 in rat basophilic leukemia mast cells and Jurkat T cells._x000B_J Immunol. 2002 May 1;168(9):4612-9. _x000B__x000B_7: Kruger JM, Fukushima T, Cherepanov V, Borregaard N, Loeve C, Shek C, Sharma K, Tanswell AK, Chow CW, Downey GP. Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides._x000B_J Biol Chem. 2002 Jan 25;277(4):2620-8. Epub 2001 Nov 15. _x000B__x000B__x000B_
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Test
  You can block the antibody by the specific target amino acid sequence of peptide.
• Properties
  blocking peptide
• Description
  Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.

• Gene target
  GPR4  
• Gene symbol
  GPR4
• Short name
  GPR4 Blocking peptide
• Technique
  blocking peptide, Blocking, peptide, blocking peptides, nordc made this blocking amino acid sequence or peptide to block the gene target in a volume of 1. For western blot it is often requested to block your antibody and to see the band of the analyzed protein disappear. This is called a negative control by blocking the WB antibody. peptides
• Isotype
  IgG
• Label
  unlabeled
• Alternative name
  GPR4 inhibiting short protein sequence
• Alternative technique
  control, peptides

Gene info

• Identity
  HGNC:4497
• Gene
  GPR4
• Long gene name
  G protein-coupled receptor 4
• GenBank acession
  • BC067536
• Locus
  19q13.32
• Discovery year
  1994-07-25
• Entrez gene record
  2828
• Pubmed identfication
  • 8595909
• RefSeq identity
  • NM_005282
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000182126

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