-
Category
Secondary Antibodies
-
Long description
The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin em (classes and both light chain types) including antibodies to common determinants, to s and to the surface determinants of the common Fab portion, as tested by immunoelectroimmunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
-
Antibody come from
Purified normal IgG and fractions containing IgA, and IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
-
Other description
Delipidated, heat inactivated lyophilized stable whole serum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Goat anti Rabbit IgG IgA IgM (heavy and light chains) Antibody
-
Antibody is raised in
Goat
-
Antibody s reacts with
Rabbit
-
Antibody s reacts with these species
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
-
Antibody s specificity
Precipitating polyclonal Goat antiSerum to Rabbit immunoglobulins, IgG, IgA and IgM, heavy and light chains.
-
Research interest
Veterinary
-
Application
Immunoprecipitation
-
Antibody s suited for
Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:32 when tested against normal rabbit serum in agar block titration.
-
Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
-
Relevant references
no information yet
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
-
Properties
Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Gene target
-
Short name
Goat anti Rabbit IgG IgA IgM (heavy light chains)
-
Technique
Rabbit, light, anti, Goat, IgA, IgG, IgM, antibody to, goats, IgAs, IgGs, IgMs
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
-
Isotype
not specified, IgA, IgG, IgM
-
Label
unlabeled
-
Species
Goats
-
Alternative name
caprine antibody to production species: rabbit Immunoglobulin G Immunoglobulin A Immunoglobulin M (heavy and light chains)
-
Alternative technique
rabbit-anti, antibodies, caprine, immunoglobulin-a, immunoglobulins, immunoglobulin-m
-
