Goat anti Rabbit albumin antibody conjugated with FITC

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• Catalog number
  GAR/Alb/FITC
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• Size
  1 mL

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• Category
  Primary Antibodies
• Long description
  The defined antibody specificity is directed to albumin as tested against rabbit sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rabbit serum and the purified albumin. As reagent for the direct detection of albumin in rabbit cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
• Antibody come from
  Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rabbit serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Other description
  Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Goat anti Rabbit albumin antibody conjugated with FITC Antibody
• Antibody is raised in
  Goat
• Antibody s reacts with
  Rabbit
• Antibody s reacts with these species
  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
• Antibody s specificity
  Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Rabbit albumin
• Research interest
  Veterinary
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
• Antibody s suited for
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
• Storage
  The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus, Oryctolagus cuniculus
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• French translation
  anticorps

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• Gene target
  albumin   conjugated   with  
• Short name
  Goat anti Rabbit albumin antibody conjugated with FITC
• Technique
  Antibody, Rabbit, anti, FITC, Goat, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
• Isotype
  not specified
• Label
  FITC
• Species
  Goats
• Alternative name
  caprine antibody to production species: rabbit albumin (antibody to-) coupled including fluorecein
• Alternative technique
  antibodies, rabbit-anti, caprine, fluorescine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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