-
Category
Secondary Antibodies
-
Long description
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the kappa type, as well as free kappa light chains. To identify light chain type in enzyme-immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the kappa type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:50 and 1:200; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:8,000.
-
Antibody come from
A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
-
Other description
Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Goat anti Mouse Ig kappa light chains (free and bound) antibody conjugated with Horseradish peroxidase Antibody
-
Antibody is raised in
Goat
-
Antibody s reacts with
Mouse
-
Antibody s reacts with these species
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
-
Antibody s specificity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse Ig kappa chain, free and bound
-
Research interest
Veterinary
-
Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
-
Antibody s suited for
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
-
Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years reconstituted at or below -20° C--3-5 years reconstituted at +4° C--7 days
-
Relevant references
no information yet
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Latin name
Capra aegagrus hircus, Mus musculus
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
-
French translation
anticorps
-
Gene target
-
Short name
Goat anti Mouse Ig kappa light chains (free bound) antibody conjugated with Horseradish
-
Technique
anti mouse, Antibody, light, Mouse, anti, Goat, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, goats, mouses
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
not specified
-
Label
HRP
-
Species
Mouse, Goats, Mouses
-
Alternative name
caprine antibody to Mouse Ig kappa light chains (free and bound) (antibody to-) coupled including Horseradish peroxidase
-
Alternative technique
antibodies, murine, caprine
-
