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Category
Primary Antibodies
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Long description
The reactivity of the antiserum is restricted to thyroxine binding globulin. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified thyroxine binding globulin. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of thyroxine binding globulin in human plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use in non-precipitating antibody-binding techniques if proper controls are included.
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Antibody come from
Thyroxine binding is a protein with a molecular weight of 55,700 kD. The protein is binding thyroxine as well as triiodothyronine. In immunoelectrophoresis it has a alpha mobility. The mean concentration in normal human serum is about 40 μg/ml. Elevated levels are observed during oestrogen administration, pregnancy hypothyroidism. Reduced levels are observed in hypothyroidism. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Goat anti Human thyroxine binding globulin Antibody
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Antibody is raised in
Goat
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Antibody s reacts with
Human
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Antibody s reacts with these species
The antiSerum does not cross react with any other component of Human plasma. Interspecies cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Goat antiSerum to Human thyroxine binding globulin
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Research interest
Hormones
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Application
Immunoprecipitation
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Antibody s suited for
Immunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:16 when tested against pooled normal human plasma in agar-block immunodiffusion titration.
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Storage
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant references
no information yet
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Protein number
P05543
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin name
Capra aegagrus hircus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
