Goat anti Human IgM (Fc specific) antibody conjugated with FITC
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Catalog numberFGAHu/IgM(Fc)/FITC
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Price:
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Size1 mL
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CategorySecondary Antibodies
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Long descriptionThe antiserum is reacting with to the Fc subunit of IgM. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
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Antibody come fromPurified IgM prepared from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionFITC-coupled goat F(ab’)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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ClonePolyclonal
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Antigen antibody binding interactionGoat anti Human IgM (Fc specific) antibody conjugated with FITC Antibody
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Antibody is raised inGoat
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Antibody s reacts withHuman
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Antibody s reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail.
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Antibody s specificityFluorescein isothiocyanate-conjugated F(ab’)2 fragments of polyclonal Goat IgG antibodies to Human IgM, Fc specific
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Research interestImmunology
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ApplicationELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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Antibody s suited forELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years reconstituted at or below -20° C--3-5 years reconstituted at +4° C--7 days
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus
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French translationanticorps
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Gene target
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Short nameGoat anti IgM (Fc specific) antibody conjugated with FITC
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Techniqueanti human, Antibody, anti, FITC, Goat, IgM, FC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats, IgMs
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotypenot specified, IgM
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LabelFITC
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SpeciesHuman, Goats, Humans
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Alternative namecaprine antibody to H. sapiens Immunoglobulin M (fragment c specific) (antibody to-) coupled including fluorecein
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Alternative techniqueantibodies, caprine, fluorescine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data