Goat anti Human albumin
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Catalog numberGAHu/Alb/7S
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Price:
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Size10 mg
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CategoryPrimary Antibodies
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Long descriptionThe defined antibody specificity is directed to albumin as tested against human sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:10,000.
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Antibody come fromAlbumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macroheterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionPurified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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ClonePolyclonal
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Antigen antibody binding interactionGoat anti Human albumin Antibody
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Antibody is raised inGoat
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Antibody s reacts withHuman
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Antibody s reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has not been tested in detail.
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Antibody s specificityPurified IgG fraction of polyclonal Goat antiSerum to Human albumin
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Research interestCardiovascular
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ApplicationIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting
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Antibody s suited forIndirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years reconstituted at or below -20° C--3-5 years reconstituted at +4° C--7 days
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Relevant referencesno information yet
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Protein numberP02768
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin nameCapra aegagrus hircus
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolALB, DBP, HNF1A
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Short nameGoat anti albumin
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Techniqueanti human, anti, Goat, antibody to, anti human antibodies, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotypenot specified
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Labelunlabeled
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SpeciesHuman, Goats, Humans
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Alternative namecaprine antibody to H. sapiens albumin
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Alternative techniqueantibodies, caprine
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Gene info
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Identity
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Gene
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Long gene namealbumin
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameD-box binding PAR bZIP transcription factor
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Synonyms gene name
- D site of albumin promoter (albumin D-box) binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PAR bZIP family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHNF1 homeobox A
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Synonyms gene
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Synonyms gene name
- transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HNF class homeoboxes
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data