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Category
Primary Antibodies
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Long description
Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
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Antibody come from
The anti-GFP antibody is a chicken polyclonal antiserum obtained from whole egg yolk of chickens immunised with highly purified native GFP derived from Aequorea victoria jellyfish.
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Other description
Each vial contains 100µl 1 mg/ml polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0,15 mM NaCl and 0.02% sodium azide. The antibodies were purified by ammonium sulfate precipitation.
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Clone
Polyclonal
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Antigen antibody binding interaction
Chicken anti-native Green Fluorescent Protein Antibody
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Antibody is raised in
Chicken
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Antibody s reacts with
n/a
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Antibody s reacts with these species
Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.
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Antibody s specificity
The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
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Application
Western Blotting, ELISA
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Antibody s suited for
The antibody is suitable for the detection of GFP by western blotting and ELISA. Optimal antibody dilutions for the different applications should be determined by titration. Suggested dilutions for immunoblotting: 1:1000-1:5000, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. For ELISA: 1:2,000 – 1:10,000 using alkaline phosphate and nitroblue tetrazolium assay.
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Storage
Store at 4oC, or in small aliquots at -20oC.
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Relevant references
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805. 2. UniProt: P42212 (A.victoria)
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Protein number
UniProt: P42212 (A.victoria)
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
