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Category
Proteins & Peptides
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Long description
As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of BSA/FITC. The purpose of counterstaining is to enhance the optical contrast of the immunospecific staining against a counterstained background.
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Antibody come from
n/a
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Other description
Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2).
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Clone
not specified
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Antigen antibody binding interaction
Bovine Serum albumin Fr.V antibody conjugated with FITC
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Antibody is raised in
Bovine
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Fluorescein isothiocyanate-conjugated Bovine Serum albumin, Cohn fraction V.
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Research interest
Cardiovascular
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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Antibody s suited for
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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About
Serum should be stored at +5°C
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French translation
anticorps