3T3-L1

Product Type

Cell Lysate
Concentration

2mg/ml (determined by BCA assay)
Formulation

Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Applications

WB
WB Comment and Pic

All lanes : 3T3-L1 Whole Cell Lysate (40ug);;Lane 1. : Anti-GAPDH antibody (ATGA0181);;Lane 2. : Anti-Beta tubulin antibody (ATGA0196) {ATGL0021-WB.jpg}
Lysate QC

Western Blot analyzed by both GAPDH and Beta tubulin antibody
Antigen Species

Mouse
Preparation before usage

Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Storage and shipping

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Data sheet

Gene target

3T3-L1
Gene symbol

MTA2, MICALL1, IGKV1-16, ZBTB4, UCHL1, COLEC10, FAM71F1, KLK4, PTGES, MCOLN1
Short name

3T3-L1
Alternative name

3T3-L1

Identity

HGNC:7411
Gene

MTA2
Long gene name

metastasis associated 1 family member 2
Synonyms gene
- MTA1L1
Synonyms gene name
- metastasis associated gene family, member 2
Synonyms
- MTA1-L1
GenBank acession
- AB016591
Locus

11q12.3
Discovery year

1999-05-17
Entrez gene record

9219
Pubmed identfication
- 9929979
RefSeq identity
- NM_004739
Classification
- GATA zinc finger domain containing
- NuRD complex subunits
- Myb/SANT domain containing
VEGA ID

OTTHUMG00000167684

Identity

HGNC:12513
Gene

UCHL1
Long gene name

ubiquitin C-terminal hydrolase L1
Synonyms gene
- PARK5
Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms
- PGP9.5
- Uch-L1
Synonyms name
- ubiquitin thiolesterase
GenBank acession
- BC000332
Locus

4p13
Discovery year

1991-07-15
Entrez gene record

7345
Pubmed identfication
- 1840236
RefSeq identity
- NM_004181
Classification
- Ubiquitin C-terminal hydrolases
VEGA ID

OTTHUMG00000099377

Identity

HGNC:2220
Gene

COLEC10
Long gene name

collectin subfamily member 10
Synonyms gene name
- collectin sub-family member 10 (C-type lectin)
Synonyms
- CL-L1
- CL-10
GenBank acession
- AB002631
Locus

8q24.12
Discovery year

2000-04-11
Entrez gene record

10584
Pubmed identfication
- 10224141
Classification
- Collectins
- C-type lectin domain containing
- Complement system activation components
VEGA ID

OTTHUMG00000164971

Identity

HGNC:30704
Gene

FAM71F1
Long gene name

family with sequence similarity 71 member F1
Synonyms gene
- FAM137A
Synonyms gene name
- family with sequence similarity 137, member A
- family with sequence similarity 71, member F1
Synonyms
- NYD-SP18
- GARI-L1
Synonyms name
- Golgi-associated RAB2B interactor like 1
GenBank acession
- AF367470
Locus

7q32.1
Discovery year

2007-07-24
Entrez gene record

84691
Pubmed identfication
- 12477932
- 34714330
RefSeq identity
- NM_032599
VEGA ID

OTTHUMG00000158276