WISP1 Antibody

• Catalog number
  R31201
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  WISP1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the WISP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This WISP1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O95388
• Purity
  Antigen affinity
• Description
  WNT1-Inducible Signaling Pathway Protein 1, also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. It is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed(Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al.(1998) mapped the gene to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al.(1998) found that two distinct systems demonstrated that WISP1 induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
• Immunogen
  An amino acid sequence from the C-terminus of human WNT1-Inducible Signaling Pathway Protein 1 (ADLESYPDFSEIAN) was used as the immunogen for this WISP1 antibody.
• Storage
  After reconstitution, the WISP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  WISP1  
• Gene symbol
  CCN4
• Short name
  Anti-WISP1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to WISP1
• Alternative technique
  antibodies
• Alternative to gene target
  WNT1 inducible signaling pathway protein 1, WISP1 and IDBG-36325 and ENSG00000104415 and 8840, insulin-like growth factor binding, Extracellular, Wisp1 and IDBG-146803 and ENSMUSG00000005124 and 22402, WISP1 and IDBG-644060 and ENSBTAG00000000707 and 539163

Gene info

• Identity
  HGNC:12769
• Gene
  CCN4
• Long gene name
  cellular communication network factor 4
• Synonyms gene
  • WISP1-OT1
  • WISP1-UT1
  • WISP1
• Synonyms gene name
  • WISP1 overlapping transcript 1
  • WISP1 3'UTR-associated RNA 1
  • WISP1 overlapping transcript 1 (non-protein coding)
  • WNT1 inducible signaling pathway protein 1
• Synonyms
  • WISP-1
• GenBank acession
  • AF100779
• Locus
  8q24.22
• Discovery year
  1999-01-22
• Entrez gene record
  8840
• Pubmed identfication
  • 9843955
• RefSeq identity
  • NM_003882
• Classification
  • Cellular communication network factors
• VEGA ID
  OTTHUMG00000164440

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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