VAChT Antibody / Vesicular acetylcholine transporter

• Catalog number
  R32623
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  VAChT / Vesicular acetylcholine transporter
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  Optimal dilution of the VAChT antibody should be determined by the researcher.
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q16572
• Purity
  Antigen affinity
• Description
  The Vesicular acetylcholine transporter (VAChT), also known as SLC18A3, is a neurotransmitter transporter which is responsible for loadingacetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
• Immunogen
  Amino acids 1-36 (MESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVL-human) were used as the immunogen for the VAChT antibody.
• Storage
  After reconstitution, the VAChT antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  VAChT   Vesicular   acetylcholine   transporter  
• Gene symbol
  SLC18A3
• Short name
  Anti- VAChT / Vesicular acetylcholine transporter
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to VAChT / Vesicular acetylcholine transporter
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10936
• Gene
  SLC18A3
• Long gene name
  solute carrier family 18 member A3
• Synonyms gene name
  • solute carrier family 18 (vesicular acetylcholine transporter), member 3
• Synonyms
  • VACHT
• GenBank acession
  • BC007765
• Locus
  10q11.23
• Discovery year
  1995-06-01
• Entrez gene record
  6572
• Pubmed identfication
  • 8071310
• RefSeq identity
  • NM_003055
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000018196

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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