V5 Tag Antibody

• Catalog number
  F52064-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  V5 Tag
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000
• Notes
  Titration of the V5 Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This V5 Tag antibodyis to be used only for research purposes and not for diagnostics..
• Purity
  Purified
• Description
  Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
• Immunogen
  The V5 sequence GKPIPNPLLGLDST was used as the immunogen for the V5 tag antibody.
• Storage
  Aliquot the V5 Tag antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Tag  
• Short name
  Anti-V5 Tag
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Isotype
  Rabbit Ig
• Label
  tag
• Alternative name
  Antibodies to V5 Tag
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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