UCP1 Antibody

• Catalog number
  R31088
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  UCP1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the UCP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This UCP1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P04633
• Purity
  Antigen affinity
• Description
  Uncoupling Protein 1, also called Thermogenin or UCP, is an uncoupling protein found in the mitochondria of brown adipose tissue(BAT). Using in situ hybridization, Cassard et al.(1990) assigned the human UCP gene to 4q31. They found that the primary structure of UCP is similar to that of ADP/ATP translocator of skeletal muscle, the gene for which is also located on chromosome 4. The failure of UCP1 expressed in E. coli inclusion bodies to carry out fatty acid-dependent H+ transport activity inclusion bodies made Echtay et al.(2000) seek a native UCP cofactor. They identified coenzyme Q(CoQ, or ubiquinone) as such a cofactor. On addition of CoQ(10) to reconstituted UCP1 from inclusion bodies, fatty acid-dependent proton transport reached the same rate as with native UCP1.
• Immunogen
  An amino acid sequence from the C-terminus of mouse Uncoupling Protein 1 (FEQLKKELMKSRQTVDCTT) was used as the immunogen for this UCP1 antibody (100% rat homology).
• Storage
  After reconstitution, the UCP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UCP1  
• Gene symbol
  UCP1
• Short name
  Anti-UCP1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to UCP1
• Alternative technique
  antibodies
• Alternative to gene target
  uncoupling protein 1 (mitochondrial, proton carrier), SLC25A7 and UCP, UCP1 and IDBG-38859 and ENSG00000109424 and 7350, oxidative phosphorylation uncoupler activity, Plasma membranes, Ucp1 and IDBG-171838 and ENSMUSG00000031710 and 22227, UCP1 and IDBG-629680 and ENSBTAG00000004647 and 101910176,281561

Gene info

• Identity
  HGNC:12517
• Gene
  UCP1
• Long gene name
  uncoupling protein 1
• Synonyms gene
  • UCP
• Synonyms gene name
  • uncoupling protein 1 (mitochondrial, proton carrier)
• Synonyms
  • SLC25A7
• GenBank acession
  • X51955
• Locus
  4q31.1
• Discovery year
  1991-08-19
• Entrez gene record
  7350
• Pubmed identfication
  • 2380264
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000133415

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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