UBE2V1 Antibody

• Catalog number
  F40115-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  UBE2V1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000
• Notes
  Titration of the UBE2V1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This UBE2V1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q13404
• Purity
  Purified
• Description
  The CROC1 isoforms, also known as UBE2V1, show sequence similarity to ubiquitin-conjugating enzymes (UBCs, or E2s) but lack the conserved cysteine residue critical to catalytic activity of E2s.1 Northern blot analysis detected approximately 2.1- and 2.5-kb CROC1 transcripts in all human tissues examined, with the highest levels in brain, skeletal muscle, and kidney. Partial human intestinal epithelial cell cDNAs have been isolated containing the 3-primecoding sequence and 3-prime untranslated region of UBE2V1, also called UEV1.2 UEV1 does not have ubiquitin-conjugating activity in vitro. UEV1 transcripts are downregulated upon differentiation of a colon carcinoma cell line.1 Constitutive expression of exogenous UEV1 protein in these colon carcinoma cells inhibits their capacity to differentiate upon confluence and induces changes in cell cycle behavior associated with inhibition of CDK1. A heterodimeric protein complex has been identified that links TRAF6 to IKK activation.3 Peptide mass fingerprinting analysis revealed that this complex is composed of the ubiquitin conjugating enzyme UBC13 and the UBC-like protein UBE2V1, also called UEV1A. TRAF6, a RING domain protein, functions together with UBC13/UEV1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains.
• Immunogen
  A portion of amino acids 113-145 from the human protein was used as the immunogen for this UBE2V1 antibody.
• Storage
  Aliquot the UBE2V1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UBE2V1  
• Gene symbol
  PEDS1-UBE2V1, UBE2V1
• Short name
  Anti-UBE2V1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to UBE2V1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:33521
• Gene
  PEDS1-UBE2V1
• Long gene name
  PEDS1-UBE2V1 readthrough
• Synonyms gene
  • TMEM189-UBE2V1
• Synonyms gene name
  • TMEM189-UBE2V1 readthrough
• Synonyms
  • Kua-UEV
  • CROC-1B
• GenBank acession
  • U39361
• Locus
  20q13.13
• Discovery year
  2007-07-31
• Entrez gene record
  387522
• Pubmed identfication
  • 11076860
• VEGA ID
  OTTHUMG00000033085

Gene info

• Identity
  HGNC:12494
• Gene
  UBE2V1
• Long gene name
  ubiquitin conjugating enzyme E2 V1
• Synonyms gene
  • UBE2V
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2 variant 1
• Synonyms
  • UEV-1
  • CROC-1
  • UEV1A
  • CROC1
• GenBank acession
  • U39360
• Locus
  20q13.13
• Discovery year
  1998-03-20
• Entrez gene record
  7335
• Pubmed identfication
  • 9418904
  • 9305758
• RefSeq identity
  • NM_021988
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000152626

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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