TRIM28 Antibody / KAP1

• Catalog number
  R32210
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  TRIM28 / KAP1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the TRIM28 antibody should be determined by the researcher.
• Intented use
  This TRIM28 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q13263
• Purity
  Antigen affinity
• Description
  Tripartite motif-containing 28, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
• Immunogen
  Amino acids 699-835 of human TRIM28 were used as the immunogen for the TRIM28 antibody.
• Storage
  After reconstitution, the TRIM28 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Nuclear
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  TRIM28   KAP1  
• Gene symbol
  TRIM28
• Short name
  Anti-TRIM28 / KAP1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to TRIM28 / KAP1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16384
• Gene
  TRIM28
• Long gene name
  tripartite motif containing 28
• Synonyms gene name
  • tripartite motif-containing 28
• Synonyms
  • TIF1B
  • KAP1
  • TF1B
  • RNF96
  • PPP1R157
• Synonyms name
  • protein phosphatase 1, regulatory subunit 157
• Locus
  19q13.43
• Discovery year
  2001-08-10
• Entrez gene record
  10155
• Pubmed identfication
  • 11331580
  • 11226167
• RefSeq identity
  • NM_005762
• Classification
  • PHD finger proteins
  • Protein phosphatase 1 regulatory subunits
  • MicroRNA protein coding host genes
  • Ring finger proteins
  • Bromodomain containing
  • Tripartite motif containing
• VEGA ID
  OTTHUMG00000183546

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products