TRAM Antibody

• Catalog number
  R31117
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  TRAM
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the TRAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This TRAM antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q15629
• Purity
  Antigen affinity
• Description
  By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that the protein influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
• Immunogen
  An amino acid sequence from the C-terminus of human Translocating chain-associating membrane protein 1 (KFINFQLRRWREHSAFQA) was used as the immunogen for this TRAM antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the TRAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  TRAM  
• Gene symbol
  TRAM1, TICAM2, NCOA3
• Short name
  Anti-TRAM
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to TRAM
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:20568
• Gene
  TRAM1
• Long gene name
  translocation associated membrane protein 1
• Synonyms
  • TRAM
  • TRAMP
• GenBank acession
  • X63679
• Locus
  8q13.3
• Discovery year
  2003-04-03
• Entrez gene record
  23471
• Pubmed identfication
  • 1315422
• RefSeq identity
  • NM_014294
• Classification
  • TLC domain containing
• VEGA ID
  OTTHUMG00000164428

Gene info

• Identity
  HGNC:21354
• Gene
  TICAM2
• Long gene name
  toll like receptor adaptor molecule 2
• Synonyms gene name
  • toll-like receptor adaptor molecule 2
• Synonyms
  • TRAM
  • TICAM-2
  • TIRP
• GenBank acession
  • AY232653
• Locus
  5q22.3
• Discovery year
  2004-07-02
• Entrez gene record
  353376
• Pubmed identfication
  • 14519765
• RefSeq identity
  • NM_021649
• Classification
  • TIR domain containing
• VEGA ID
  OTTHUMG00000162905

Gene info

• Identity
  HGNC:7670
• Gene
  NCOA3
• Long gene name
  nuclear receptor coactivator 3
• Synonyms
  • RAC3
  • AIB1
  • ACTR
  • p/CIP
  • TRAM-1
  • CAGH16
  • TNRC16
  • KAT13B
  • bHLHe42
  • SRC-3
  • SRC3
• Synonyms name
  • receptor-associated coactivator 3
  • thyroid hormone receptor activator molecule 1
• GenBank acession
  • AF012108
• Locus
  20q13.12
• Discovery year
  1999-12-17
• Entrez gene record
  8202
• Pubmed identfication
  • 9252329
  • 9346901
• RefSeq identity
  • NM_006534
• Classification
  • Basic helix-loop-helix proteins
  • Lysine acetyltransferases
• VEGA ID
  OTTHUMG00000033061

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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