Tollip Antibody

• Catalog number
  R31112
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Tollip
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Tollip antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Tollip antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9H0E2
• Purity
  Antigen affinity
• Description
  Toll-Interacting Protein is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al.(2009) stated that the gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al.(2000) confirmed the binding of Tollip to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al.(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by Tollip-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of Tollip from IRAK, which can then transmit the IL1-induced signals.
• Immunogen
  An amino acid sequence from the C-terminus of human Toll-Interacting Protein (NAQPRCSEEDLKAIQDMFPN) was used as the immunogen for this Tollip antibody.
• Storage
  After reconstitution, the Tollip antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Tollip  
• Gene symbol
  TOLLIP-DT, TOLLIP
• Short name
  Anti-Tollip
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Tollip
• Alternative technique
  antibodies
• Alternative to gene target
  toll interacting protein, TOLLIP and IDBG-19140 and ENSG00000078902 and 54472, interleukin-1, nuclei, Tollip and IDBG-212409 and ENSMUSG00000025139 and 54473, TOLLIP and IDBG-645625 and ENSBTAG00000008237 and 539480

Gene info

• Identity
  HGNC:27403
• Gene
  TOLLIP-DT
• Long gene name
  TOLLIP divergent transcript
• Synonyms gene
  • TOLLIP-AS1
• Synonyms gene name
  • TOLLIP antisense RNA 1 (head to head)
• Locus
  11p15.5
• Discovery year
  2013-05-31
• Entrez gene record
  255512
• Pubmed identfication
  • 12477932
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000166230

Gene info

• Identity
  HGNC:16476
• Gene
  TOLLIP
• Long gene name
  toll interacting protein
• Synonyms
  • IL-1RAcPIP
• GenBank acession
  • AJ242972
• Locus
  11p15.5
• Discovery year
  2003-03-13
• Entrez gene record
  54472
• Pubmed identfication
  • 9426216
  • 10854325
• RefSeq identity
  • NM_019009
• Classification
  • C2 domain containing
• VEGA ID
  OTTHUMG00000133333

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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