TBP Antibody TATA Binding Protein

• Catalog number
  R30610
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  TBP TATA Binding Protein
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This TBP antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P20226
• Purity
  Antigen affinity
• Description
  TATA-binding protein is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably only one of the proteins involved in positioning RNA polymerase II.
• Immunogen
  An amino acid sequence from the middle region of human TATA binding protein (EEQSRLAARKYARVVQKLGF) was used as the immunogen for this TBP antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the TBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  TBP   TATA   Binding   Protein  
• Gene symbol
  TBP, PSMC3, PSMC4
• Short name
  Anti-TBP TATA Binding Protein
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to TBP TATA Binding Protein
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:11588
• Gene
  TBP
• Long gene name
  TATA-box binding protein
• Synonyms gene
  • GTF2D1
  • SCA17
• Synonyms
  • TFIID
• GenBank acession
  • M55654
• Locus
  6q27
• Discovery year
  1993-05-26
• Entrez gene record
  6908
• Pubmed identfication
  • 2194289
  • 11448935
• RefSeq identity
  • NM_003194
• Classification
  • General transcription factor IID complex subunits
  • General transcription factor IIIB complex subunits
  • General transcription factors
• VEGA ID
  OTTHUMG00000016084

Gene info

• Identity
  HGNC:9549
• Gene
  PSMC3
• Long gene name
  proteasome 26S subunit, ATPase 3
• Synonyms gene name
  • proteasome (prosome, macropain) 26S subunit, ATPase, 3
• Synonyms
  • TBP1
  • TBP-1
  • RPT5
• GenBank acession
  • M34079
• Locus
  11p11.2
• Discovery year
  1995-12-06
• Entrez gene record
  5702
• Pubmed identfication
  • 9048938
  • 9473509
• RefSeq identity
  • NM_002804
• Classification
  • Proteasome
  • AAA ATPases
• VEGA ID
  OTTHUMG00000167692

Gene info

• Identity
  HGNC:9551
• Gene
  PSMC4
• Long gene name
  proteasome 26S subunit, ATPase 4
• Synonyms gene
  • MIP224
• Synonyms gene name
  • proteasome (prosome, macropain) 26S subunit, ATPase, 4
• Synonyms
  • TBP7
  • S6
  • MGC8570
  • MGC13687
  • MGC23214
  • TBP-7
  • RPT3
• Synonyms name
  • protease 26S subunit 6
  • Tat-binding protein 7
  • MB67 interacting protein
• GenBank acession
  • U27515
• Locus
  19q13.11-q13.13
• Discovery year
  1995-12-06
• Entrez gene record
  5704
• Pubmed identfication
  • 9473509
  • 8603043
• RefSeq identity
  • NM_006503
• Classification
  • AAA ATPases
  • Proteasome
• VEGA ID
  OTTHUMG00000182577

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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