SPARCL1 Antibody

• Catalog number
  R31200
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SPARCL1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the SPARCL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SPARCL1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q14515
• Purity
  Antigen affinity
• Description
  SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules(HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration(Girard and Springer, 1995).
• Immunogen
  An amino acid sequence from the C-terminus of human SPARC-Like Protein 1 (HCFGIKEEDIDENLLF) was used as the immunogen for this SPARCL1 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the SPARCL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SPARCL1  
• Gene symbol
  SPARCL1
• Short name
  Anti-SPARCL1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to SPARCL1
• Alternative technique
  antibodies
• Alternative to gene target
  SPARC-like 1 (hevin), MAST 9 and MAST9 and PIG33 and SC1, SPARCL1 and IDBG-29103 and ENSG00000152583 and 8404, protein binding, Extracellular, Sparcl1 and IDBG-185288 and ENSMUSG00000029309 and 13602, SPARCL1 and IDBG-643320 and ENSBTAG00000004094 and 507537

Gene info

• Identity
  HGNC:11220
• Gene
  SPARCL1
• Long gene name
  SPARC like 1
• Synonyms gene name
  • SPARC-like 1 (mast9, hevin)
• Synonyms
  • MAST9
• Synonyms name
  • hevin
• GenBank acession
  • X86693
• Locus
  4q22.1
• Discovery year
  1998-09-17
• Entrez gene record
  8404
• Pubmed identfication
  • 8488563
  • 7600298
  • 16844696
• Classification
  • SPARC family
• VEGA ID
  OTTHUMG00000130605

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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