SPARC Antibody

• Catalog number
  F49838-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SPARC
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, FACS, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
• Notes
  Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SPARC antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P09486
• Purity
  Purified
• Description
  Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
• Immunogen
  A portion of amino acids 224-251 from the human protein was used as the immunogen for this SPARC antibody.
• Storage
  Aliquot the SPARC antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SPARC  
• Gene symbol
  CLMAT3, SPARC
• Short name
  Anti-SPARC
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to SPARC
• Alternative technique
  antibodies
• Alternative to gene target
  secreted protein, acidic, cysteine-rich (osteonectin), ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, extracellular matrix binding, nuclei, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077

Gene info

• Identity
  HGNC:52287
• Gene
  CLMAT3
• Long gene name
  colorectal liver metastasis associated transcript 3
• Synonyms
  • SPARC-AS1
• Synonyms name
  • SPARC antisense RNA 1
• Locus
  5q33.1
• Discovery year
  2017-06-15
• Entrez gene record
  101927096
• Pubmed identfication
  • 26050227
• RefSeq identity
  • NR_109873
• Classification
  • Long non-coding RNAs with non-systematic symbols

Gene info

• Identity
  HGNC:11219
• Gene
  SPARC
• Long gene name
  secreted protein acidic and cysteine rich
• Synonyms gene
  • ON
• Synonyms gene name
  • osteonectin
• Synonyms
  • ONT
  • BM-40
• Synonyms name
  • cysteine-rich protein
  • secreted protein acidic and rich in cysteine
• Locus
  5q33.1
• Discovery year
  2001-06-22
• Entrez gene record
  6678
• Pubmed identfication
  • 2838412
  • 3410046
• RefSeq identity
  • NM_003118
• Classification
  • SPARC family
• VEGA ID
  OTTHUMG00000130122

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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