SDHA Antibody

• Catalog number
  R30363
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.2mg/ml in 1X PBS with 50% glycerol and 0.025% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SDHA
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the SDHA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SDHA antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P31040
• Purity
  Antigen affinity
• Description
  Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit(SDHA), the iron sulfur protein subunit(SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase(SDH) protein complex catalyzes the oxidation of succinate(succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes(Types I and II) in the paraganglia system.
• Immunogen
  An amino acid sequence from the C-terminus of human SDHA (YRPVIDKTLNEADCAT) was used as the immunogen for this SDHA antibody (100% homologous in human, mouse and rat).
• Storage
  Store the SDHA antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SDHA  
• Gene symbol
  SDHA
• Short name
  Anti-SDHA
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to SDHA
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10680
• Gene
  SDHA
• Long gene name
  succinate dehydrogenase complex flavoprotein subunit A
• Synonyms gene
  • SDH2
• Synonyms gene name
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
  • succinate dehydrogenase complex subunit A, flavoprotein (Fp)
• Synonyms
  • FP
  • SDHF
• Synonyms name
  • succinate dehydrogenase [ubiquinone] flavoprotein subunit
  • flavoprotein subunit of complex II
• GenBank acession
  • BC001380
• Locus
  5p15.33
• Discovery year
  1995-10-24
• Entrez gene record
  6389
• Pubmed identfication
  • 7798181
• RefSeq identity
  • NM_004168
• Classification
  • Mitochondrial complex II: succinate dehydrogenase subunits
• VEGA ID
  OTTHUMG00000090275
• Locus Specific Databases
  • SDHA @ LOVD
  • Global Variome shared LOVD
  • LRG_315

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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