S100 beta Antibody

• Catalog number
  R30360
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  S100 beta
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the S100 beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This S100 beta antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P04271
• Purity
  Antigen affinity
• Description
  S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.
• Immunogen
  An amino acid sequence from the C-terminus of human S100 beta (DGDGECDFQEFMAFV) was used as the immunogen for this S100 beta antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the S100 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  S100   beta  
• Gene symbol
  S100A1, S100Z, S100A8, S100A9
• Short name
  Anti-S100 beta
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to S100 beta
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10486
• Gene
  S100A1
• Long gene name
  S100 calcium binding protein A1
• Synonyms gene
  • S100A
• Synonyms gene name
  • S100 calcium-binding protein A1
• Synonyms
  • S100-alpha
• GenBank acession
  • BC014392
• Locus
  1q21.3
• Discovery year
  1991-07-10
• Entrez gene record
  6271
• Pubmed identfication
  • 1998503
• RefSeq identity
  • NM_006271
• Classification
  • S100 calcium binding proteins
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000037041

Gene info

• Identity
  HGNC:30367
• Gene
  S100Z
• Long gene name
  S100 calcium binding protein Z
• Synonyms gene name
  • S100 calcium binding protein, zeta
• Synonyms
  • Gm625
  • S100-zeta
• GenBank acession
  • AF437876
• Locus
  5q13.3
• Discovery year
  2004-09-22
• Entrez gene record
  170591
• Pubmed identfication
  • 11747429
• RefSeq identity
  • NM_130772
• Classification
  • S100 calcium binding proteins
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000162441

Gene info

• Identity
  HGNC:10498
• Gene
  S100A8
• Long gene name
  S100 calcium binding protein A8
• Synonyms gene
  • CAGA
  • CFAG
• Synonyms gene name
  • calgranulin A
  • cystic fibrosis antigen
• Synonyms
  • P8
  • MRP8
  • MRP-8
  • 60B8AG
  • CGLA
  • S100-A8
• Synonyms name
  • Migration inhibitory factor-related protein 8
  • Calprotectin L1L subunit
  • Leukocyte L1 complex light chain
• GenBank acession
  • BC005928
• Locus
  1q21.3
• Discovery year
  1989-05-19
• Entrez gene record
  6279
• RefSeq identity
  • NM_002964
• Classification
  • S100 calcium binding proteins
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000013124

Gene info

• Identity
  HGNC:10499
• Gene
  S100A9
• Long gene name
  S100 calcium binding protein A9
• Synonyms gene
  • CAGB
  • CFAG
• Synonyms gene name
  • calgranulin B
  • S100 calcium-binding protein A9 (calgranulin B)
• Synonyms
  • P14
  • MIF
  • NIF
  • LIAG
  • MRP14
  • MAC387
  • 60B8AG
  • CGLB
  • MRP-14
  • S100-A9
• Synonyms name
  • Calprotectin L1H subunit
  • Leukocyte L1 complex heavy chain
  • Migration inhibitory factor-related protein 14
• GenBank acession
  • BC047681
• Locus
  1q21.3
• Discovery year
  1989-05-19
• Entrez gene record
  6280
• RefSeq identity
  • NM_002965
• Classification
  • S100 calcium binding proteins
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000013125

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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