PP2Ac Antibody
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Catalog numberR30249
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenPP2Ac
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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NotesVariations in secondary/substrate sensitivities and test protocols may require the PP2Ac antibody to be titrated for optimal performance.
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Intented useThis PP2Ac antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP67775
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PurityAntigen affinity
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DescriptionThe catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of PPP2CA is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
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ImmunogenAn amino acid sequence from the N-terminus of human PPP2CA (FTKELDQWIEQLNEC) was used as the immunogen for this PP2Ac antibody (100% homologous in human, mouse and rat).
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StorageThe lyophilized PP2Ac antibody can be stored at 4oC to -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. After reconstitution, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freeze/thaws.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPPP2CA
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Short nameAnti-PP2Ac
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to PP2Ac
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 2 catalytic subunit alpha
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Synonyms gene name
- protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
- protein phosphatase 2, catalytic subunit, alpha isozyme
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-11-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- STRIPAK complex
- Protein phosphatase catalytic subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data