PKM2 Antibody

• Catalog number
  F40140-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  PKM2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Primate, Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IF, IHC, ELISA
• Recommended dilutions
  Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:1:100
• Notes
  Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This PKM2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P14618
• Purity
  Purified
• Description
  PKM2 is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]
• Immunogen
  A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.
• Storage
  Aliquot the PKM2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PKM2  
• Gene symbol
  PKM
• Short name
  Anti-PKM2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to PKM2
• Alternative technique
  antibodies
• Alternative to gene target
  pyruvate kinase, muscle, CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, poly(A) RNA binding, nuclei, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571

Gene info

• Identity
  HGNC:9021
• Gene
  PKM
• Long gene name
  pyruvate kinase M1/2
• Synonyms gene
  • PKM2
• Synonyms gene name
  • pyruvate kinase, muscle
• Synonyms
  • THBP1
  • OIP3
  • PK3
• GenBank acession
  • M23725
• Locus
  15q23
• Discovery year
  2001-06-22
• Entrez gene record
  5315
• Pubmed identfication
  • 2040271
• VEGA ID
  OTTHUMG00000172709

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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