Phospho-GSK3B Antibody (pS21/29)
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Catalog number
F48400-0.4ML
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Price
Please ask
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Size
0.4 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Phospho-GSK3B (pS21/29)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC, ELISA
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Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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Notes
Titration of the phospho-GSK3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-GSK3B antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P49841
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Purity
Antigen affinity
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Description
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
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Immunogen
This phospho-GSK3B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS21/29 of human GSK3B.
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Storage
Aliquot the phospho-GSK3B antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
GSK3B, GSK3B-DT
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Short name
Anti-Phospho-GSK3B (pS21/29)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Phospho-GSK3B (pS21/29)
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Alternative technique
antibodies
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Alternative to gene target
glycogen synthase kinase 3 beta, GSK3B and IDBG-51663 and ENSG00000082701 and 2932, transferase activity, nuclei, Gsk3b and IDBG-159859 and ENSMUSG00000022812 and 56637, BT.48740 and IDBG-632831 and ENSBTAG00000048057 and 790875
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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