Phospho-Bad Antibody (pS118)
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Catalog number
F48366-0.4ML
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Price
Please ask
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Size
0.4 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Phospho-Bad (pS118)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC, ELISA
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Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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Notes
Titration of the phospho-Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-Bad antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q92934
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Purity
Antigen affinity
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Description
Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation
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Immunogen
This phospho-Bad antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human Bad.
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Storage
Aliquot the phospho-Bad antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Anti-Phospho-Bad (pS118)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Phospho-Bad (pS118)
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Alternative technique
antibodies
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Alternative to gene target
BCL2-associated a this GO nist of cell death, BBC2 and BCL2L8, BAD and IDBG-53423 and ENSG00000002330 and 572, protein heterodimerization activity, Cytoplasm, Bad and IDBG-137335 and ENSMUSG00000024959 and 12015, BT.55802 and IDBG-643918 and ENSBTAG00000012511 and 615013
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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