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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
p57Kip2
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the p57Kip2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This p57Kip2 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P49919
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Purity
Antigen affinity
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Description
Cyclin-dependent kinase inhibitor 1C, also called p57(KIP2) or KIP2, is a protein which in humans is encoded by the CDKN1C imprinted gene. The CDKN1C gene encodes a potent tight-binding inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. The gene is paternally imprinted, with preferential expression of the maternal allele. Matsuoka et al.(1995)demonstrated that the gene is located on chromosome 11p15.5, a region implicated in both sporadic cancers and Beckwith-Wiedemann syndrome, a familial cancer syndrome, making it a tumor suppressor candidate. The gene contains 3 exons. Mutations of p57Kip2 are implicated in sporadic cancers and Beckwith-Wiedemann syndrome suggesting that it is a tumor suppressor candidate. Diaz-Meyer et al.(2005) concluded that p57Kip2 may be downregulated by multiple mechanisms including some that do not involve promoter methylation.
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Immunogen
An amino acid sequence from the N-terminus of mouse p57 Kip2 (RLAELNAEDQNRWDFNFQQD) was used as the immunogen for this p57Kip2 antibody (100% rat homology).
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Storage
After reconstitution, the p57Kip2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
