p53R2 Antibody

• Catalog number
  R31164
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  p53R2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the p53R2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This p53R2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q7LG56
• Purity
  Antigen affinity
• Description
  Ribonucleotide Reductase, M2 B, also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al.(2000) mapped the gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al.(2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.
• Immunogen
  An amino acid sequence from the C-terminus of human p53 reductase small subunit 2 (EGKTNFFEKRVSEYQ) was used as the immunogen for this p53R2 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the p53R2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  p53R2  
• Gene symbol
  RRM2B
• Short name
  Anti-p53R2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to p53R2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17296
• Gene
  RRM2B
• Long gene name
  ribonucleotide reductase regulatory TP53 inducible subunit M2B
• Synonyms gene name
  • ribonucleotide reductase M2 B (TP53 inducible)
• Synonyms
  • p53R2
• GenBank acession
  • AB036532
• Locus
  8q22.3
• Discovery year
  2002-01-14
• Entrez gene record
  50484
• Pubmed identfication
  • 10716435
  • 10980602
  • 17486094
• VEGA ID
  OTTHUMG00000164776
• Locus Specific Databases
  • LRG_788

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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