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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
NGFR Nerve Growth Factor Receptor
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This NGFR antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P08138
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Purity
Antigen affinity
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Description
The Low-Affinity Nerve Growth Factor Receptor(also called the NGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. It is mapped to 17q21.33. Overexpression of the protein was also found to activate the SM pathway. NGFR is a receptor for rabies virus, and it is also a coreceptor for the NOGO receptor for MAG signaling. This gene is a signaling partner for EPHAs and the EPHA/NGFR complex reverse signals can mediate axon repulsion required for guidance and mapping. NGFR is involved in activity-dependent synaptic plasticity. It has been shown to interact with PRKACB, FSCN1, TRAF4, Nerve growth factor, TRAF2, NDN, NGFRAP1 and MAGEH1.
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Immunogen
An amino acid sequence from the C-terminus of human Nerve growth factor receptor (TLDALLAALRRIQRADLV) was used as the immunogen for this NGFR antibody.
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Storage
After reconstitution, the NGFR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
