NFKB2 (p100) Antibody
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Catalog numberF47541-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenNFKB2 (p100)
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, ELISA
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Recommended dilutionsWestern blot: 1:1000
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NotesTitration of the NFKB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis NFKB2 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ00653
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PurityAntigen affinity
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DescriptionNF-kappa-B has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-kappa-B is activated by a wide variety of stimuli, such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. NFKB1 (MIM 164011) and NFKB2 encode p105 and p100 proteins that are processed to produce the active p50 and p52 NF-kappa-B subunits, respectively. However, the p100 and p105 proteins serve regulatory functions and should not be considered exclusively as precursor forms. The most abundant activated form of NF-kappa-B is a heterodimer of the p50 or p52 subunit bound to the RELA subunit (MIM 164014). Other NF-kappa-B complexes, consisting of hetero- and homodimers of p50, p52, RELA, REL (MIM 164910), and RELB (MIM 604758), have also been detected. NF-kappa-B complexes are inhibited by I-kappa-B proteins, NFKBIA (MIM 164008) or NFKBIB (MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by the kinases IKBKA (CHUK; MIM 600664) or IKBKB (MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. The activated NF-kappa-B complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].
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ImmunogenA portion of amino acids 848-874 from the human protein was used as the immunogen for this NFKB2 antibody.
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StorageAliquot the NFKB2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolNFKB2
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Short nameAnti-NFKB2 (p100)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to NFKB2 (p100)
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Alternative techniqueantibodies
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Alternative to gene targetnuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), CVID10 and H2TF1 and LYT-10 and LYT10 and NF-kB2 and p100 and p52, NFKB2 and IDBG-87893 and ENSG00000077150 and 4791, protein binding, nuclei, Nfkb2 and IDBG-172544 and ENSMUSG00000025225 and 18034, BT.20288 and IDBG-639157 and ENSBTAG00000006017 and 526392
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Gene info
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Identity
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Gene
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Long gene namenuclear factor kappa B subunit 2
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Synonyms gene name
- nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-11-14
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Entrez gene record
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Pubmed identfication
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Classification
- IPT domain containing
- NF-kappa B complex subunits
- Ankyrin repeat domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data