mTOR Antibody (FRAP1)
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Catalog numberF49548-0.4ML
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PricePlease ask
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenmTOR (FRAP1)
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC, IF, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
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NotesTitration of the mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis mTOR antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP42345
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PurityAntigen affinity
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DescriptionFRAP1/mTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. It is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
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ImmunogenA portion of amino acids 2459-2488 from the human protein was used as the immunogen for this mTOR antibody.
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StorageAliquot the mTOR antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMTOR
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Short nameAnti-mTOR (FRAP1)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to mTOR (FRAP1)
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Alternative techniqueantibodies
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Alternative to gene targetmechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219
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Gene info
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Identity
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Gene
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Long gene namemechanistic target of rapamycin kinase
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Synonyms gene
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Synonyms gene name
- FK506 binding protein 12-rapamycin associated protein 1
- mechanistic target of rapamycin (serine/threonine kinase)
- mechanistic target of rapamycin
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Synonyms
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Synonyms name
- FK506 binding protein 12-rapamycin associated protein 2
- rapamycin target protein
- FKBP12-rapamycin complex-associated protein 1
- FKBP-rapamycin associated protein
- rapamycin associated protein FRAP2
- dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1)
- rapamycin and FKBP12 target 1
- mammalian target of rapamycin
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GenBank acession
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Locus
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Discovery year1995-07-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MTOR complex 1
- MTOR complex 2
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data