mTOR Antibody (FRAP1)
#
-
Catalog numberF49548-0.08ML
-
Price:
-
Size0.08 ml
-
-
CategoryAntibody
-
ConcentrationIn 1X PBS pH 7.4 with 0.09% sodium azide
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenmTOR (FRAP1)
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB, IHC, IF, ELISA
-
Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
-
NotesTitration of the mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented useThis mTOR antibodyis to be used only for research purposes and not for diagnostics..
-
UniprotP42345
-
PurityAntigen affinity
-
DescriptionFRAP1/mTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. It is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
-
ImmunogenA portion of amino acids 2459-2488 from the human protein was used as the immunogen for this mTOR antibody.
-
StorageAliquot the mTOR antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolMTOR
-
Short nameAnti-mTOR (FRAP1)
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit Ig
-
Alternative nameAntibodies to mTOR (FRAP1)
-
Alternative techniqueantibodies
-
Alternative to gene targetmechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219
-
Gene info
-
Identity
-
Gene
-
Long gene namemechanistic target of rapamycin kinase
-
Synonyms gene
-
Synonyms gene name
- FK506 binding protein 12-rapamycin associated protein 1
- mechanistic target of rapamycin (serine/threonine kinase)
- mechanistic target of rapamycin
-
Synonyms
-
Synonyms name
- FK506 binding protein 12-rapamycin associated protein 2
- rapamycin target protein
- FKBP12-rapamycin complex-associated protein 1
- FKBP-rapamycin associated protein
- rapamycin associated protein FRAP2
- dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1)
- rapamycin and FKBP12 target 1
- mammalian target of rapamycin
-
GenBank acession
-
Locus
-
Discovery year1995-07-18
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MTOR complex 1
- MTOR complex 2
- Armadillo like helical domain containing
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data