MIF Antibody

• Catalog number
  R30122
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MIF
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the MIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MIF antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P14174
• Purity
  Antigen affinity
• Description
  Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1kb. Localization of the human gene for macrophage migration inhibitory factor(MIF) to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis.
• Immunogen
  Human partial recombinant protein (AA 1-115) was used as the immunogen for this MIF antibody.
• Storage
  After reconstitution, the MIF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MIF  
• Gene symbol
  MIF-AS1, MIF, ODC1-DT, DDT, S100A9
• Short name
  Anti-MIF
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to MIF
• Alternative technique
  antibodies
• Alternative to gene target
  macrophage migration inhibitory factor (glycosylation-inhibiting factor), GIF and GLIF and MMIF, MIF and IDBG-401910 and ENSG00000240972 and 4282, phenylpyruvate tautomerase activity, Extracellular, Mif and IDBG-163956 and ENSMUSG00000033307 and 17319, MIF and IDBG-637094 and ENSBTAG00000007375 and 280858

Gene info

• Identity
  HGNC:27669
• Gene
  MIF-AS1
• Long gene name
  MIF antisense RNA 1
• Synonyms
  • LOC284889
  • MIF-AS
• Locus
  22q11.23
• Discovery year
  2014-08-13
• Entrez gene record
  284889
• Pubmed identfication
  • 24066864
  • 26895959
• RefSeq identity
  • NR_038911
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000150772

Gene info

• Identity
  HGNC:7097
• Gene
  MIF
• Long gene name
  macrophage migration inhibitory factor
• Synonyms gene
  • GLIF
• Synonyms gene name
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Synonyms
  • GIF
• Synonyms name
  • glycosylation-inhibiting factor
  • phenylpyruvate tautomerase
• GenBank acession
  • M25639
• Locus
  22q11.23
• Discovery year
  1993-10-15
• Entrez gene record
  4282
• Pubmed identfication
  • 7558020
  • 2552447
• RefSeq identity
  • NM_002415
• VEGA ID
  OTTHUMG00000150773

Gene info

• Identity
  HGNC:54070
• Gene
  ODC1-DT
• Long gene name
  ODC1 divergent transcript
• Synonyms
  • lncRNA-MIF
• Synonyms name
  • c-Myc inhibitory factor
• Locus
  2p25.1
• Discovery year
  2018-10-24
• Entrez gene record
  101929715
• Pubmed identfication
  • 27317567
• RefSeq identity
  • NR_110597
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:2732
• Gene
  DDT
• Long gene name
  D-dopachrome tautomerase
• Synonyms
  • DDCT
  • MIF2
  • MIF-2
  • D-DT
• Synonyms name
  • D-dopachrome decarboxylase
• GenBank acession
  • U49785
• Locus
  22q11.23
• Discovery year
  1998-03-06
• Entrez gene record
  1652
• Pubmed identfication
  • 9480844
  • 9729470
  • 22507380
  • 29184004
• RefSeq identity
  • NM_001355
• VEGA ID
  OTTHUMG00000150798

Gene info

• Identity
  HGNC:10499
• Gene
  S100A9
• Long gene name
  S100 calcium binding protein A9
• Synonyms gene
  • CAGB
  • CFAG
• Synonyms gene name
  • calgranulin B
  • S100 calcium-binding protein A9 (calgranulin B)
• Synonyms
  • P14
  • MIF
  • NIF
  • LIAG
  • MRP14
  • MAC387
  • 60B8AG
  • CGLB
  • MRP-14
  • S100-A9
• Synonyms name
  • Calprotectin L1H subunit
  • Leukocyte L1 complex heavy chain
  • Migration inhibitory factor-related protein 14
• GenBank acession
  • BC047681
• Locus
  1q21.3
• Discovery year
  1989-05-19
• Entrez gene record
  6280
• RefSeq identity
  • NM_002965
• Classification
  • S100 calcium binding proteins
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000013125

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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