MAG Antibody
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Catalog numberR30844
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenMAG
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml (1)
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NotesThe stated application concentrations are suggested starting points. Titration of the MAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. MAG is seen in western blotting from 60~100KD depending on the level of glycosylation.
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Intented useThis MAG antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP20916
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PurityAntigen affinity
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DescriptionMyelin-associated glycoprotein, also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. Resistant embryonic neurons were rendered sensitive by expression of NgR. The glycoprotein binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with the glycoprotein prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.
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ImmunogenAmino acids 611-626 (YTLTEELAEYAEIRVK) were used as the immunogen for this MAG antibody (100% homologous in human, mouse and rat).
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StorageAfter reconstitution, the MAG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMAG, RETREG2, ERCC2
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Short nameAnti-MAG
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to MAG
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Alternative techniqueantibodies
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Alternative to gene targetmyelin associated glycoprotein, GMA and S-MAG and SIGLEC-4A and SIGLEC4A, MAG and IDBG-43939 and ENSG00000105695 and 4099, protein binding, Plasma membranes, Mag and IDBG-171670 and ENSMUSG00000036634 and 17136, BT.96871 and IDBG-635591 and ENSBTAG00000017044 and 540576
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Gene info
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Identity
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Gene
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Long gene namemyelin associated glycoprotein
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- I-set domain containing
- Sialic acid binding Ig like lectins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereticulophagy regulator family member 2
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Synonyms gene
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Synonyms gene name
- chromosome 2 open reading frame 17
- family with sequence similarity 134, member A
- family with sequence similarity 134 member A
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameERCC excision repair 2, TFIIH core complex helicase subunit
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Synonyms gene
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Synonyms gene name
- xeroderma pigmentosum complementary group D
- excision repair cross-complementing rodent repair deficiency, complementation group 2
- excision repair cross-complementation group 2
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Xeroderma pigmentosum complementation groups
- ERCC excision repair associated
- DNA helicases
- General transcription factor IIH complex subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data