Lin28a Antibody

• Catalog number
  F53240-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Lin28a
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000-2000
• Notes
  Titration of the Lin28a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Lin28a antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q8K3Y3
• Purity
  Antigen affinity
• Description
  Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, Lin28a down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'- GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
• Immunogen
  This mouse Lin28a antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 177-210 amino acids from the C-terminal region of mouse Lin28a.
• Storage
  Aliquot the Lin28a antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Lin28a  
• Gene symbol
  LIN28AP3, LIN28AP1, LIN28AP2
• Short name
  Anti-Lin28a
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Lin28a
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:38057
• Gene
  LIN28AP3
• Long gene name
  LIN28A pseudogene 3
• Synonyms gene name
  • lin-28 homolog A (C. elegans) pseudogene 3
• Locus
  20p12.2
• Discovery year
  2010-04-06
• Entrez gene record
  441940

Gene info

• Identity
  HGNC:38055
• Gene
  LIN28AP1
• Long gene name
  LIN28A pseudogene 1
• Synonyms gene name
  • lin-28 homolog A (C. elegans) pseudogene 1
• Locus
  2q32.1
• Discovery year
  2010-04-06
• Entrez gene record
  129402
• VEGA ID
  OTTHUMG00000154267

Gene info

• Identity
  HGNC:38056
• Gene
  LIN28AP2
• Long gene name
  LIN28A pseudogene 2
• Synonyms gene name
  • lin-28 homolog A (C. elegans) pseudogene 2
• Locus
  13q31.2
• Discovery year
  2010-04-06
• Entrez gene record
  100287388
• VEGA ID
  OTTHUMG00000017164

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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