LGR5 Antibody

• Catalog number
  F48166-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  LGR5
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, IF, FACS, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
• Notes
  Titration of the LGR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This LGR5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O75473
• Purity
  Purified
• Description
  LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
• Immunogen
  A portion of amino acids 238-270 from the human protein was used as the immunogen for this LGR5 antibody.
• Storage
  Aliquot the LGR5 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  LGR5  
• Gene symbol
  LGR5
• Short name
  Anti-LGR5
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to LGR5
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4504
• Gene
  LGR5
• Long gene name
  leucine rich repeat containing G protein-coupled receptor 5
• Synonyms gene
  • GPR67
  • GPR49
• Synonyms gene name
  • G protein-coupled receptor 49
• Synonyms
  • HG38
  • FEX
• GenBank acession
  • AF062006
• Locus
  12q21.1
• Discovery year
  1999-10-22
• Entrez gene record
  8549
• Pubmed identfication
  • 9642114
• RefSeq identity
  • NM_003667
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000169543

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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