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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
KLF8
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the KLF8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This KLF8 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
O95600
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Purity
Antigen affinity
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Description
Kruppel-like factor 8 is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins all contain a characteristic C-terminal DNA-binding domain containing three Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the gene to the X chromosome (WI-18614). Suske et al.(2005) stated that the human gene maps to chromosome Xp11.21, and the mouse gene to chromosome XF3. van Vliet et al.(2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser(PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.
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Immunogen
An amino acid sequence from the N-terminus of human Kruppel-like factor 8 (MVDMDKLINNLEVQLNSE) was used as the immunogen for this KLF8 antibody.
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Storage
After reconstitution, the KLF8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
