IKKb Antibody (phospho-Y199)
-
Catalog number
F48405-0.08ML
-
Price
Please ask
-
Size
0.08 ml
-
-
Category
Antibody
-
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
-
Form
Antigen affinity purified
-
Conjugation
Unconjugated
-
Clone
Polyclonal antibody
-
Recognised antigen
IKKb (phospho-Y199)
-
Host animal
Rabbit (Oryctolagus cuniculus)
-
Clonality
Polyclonal (rabbit origin)
-
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applications
WB, ELISA
-
Recommended dilutions
Western blot: 1:1000
-
Notes
Titration of the phospho-IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented use
This phospho-IKKb antibodyis to be used only for research purposes and not for diagnostics..
-
Uniprot
O14920
-
Purity
Antigen affinity
-
Description
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.
-
Immunogen
This phospho-IKKb antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY199 of human IKKb.
-
Storage
Aliquot the phospho-IKKb antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Short name
Anti-IKKb (phospho-Y199)
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Rabbit Ig
-
Alternative name
Antibodies to IKKb (phospho-Y199)
-
Alternative technique
antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products