HRH3 Antibody

• Catalog number
  R30304
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  HRH3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the HRH3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This HRH3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9Y5N1
• Purity
  Antigen affinity
• Description
  The histamine receptor H3(HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% amino acid sequence homology with the H1(HRH1) and H2(HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.
• Immunogen
  An amino acid sequence from the C-terminus of human HRH3 (CPQKLKIQPHSSLEHCWK) was used as the immunogen for this HRH3 antibody.
• Storage
  After reconstitution, the HRH3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HRH3  
• Gene symbol
  HRH3
• Short name
  Anti-HRH3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to HRH3
• Alternative technique
  antibodies
• Alternative to gene target
  histamine receptor H3, GPCR97 and HH3R, HRH3 and IDBG-84247 and ENSG00000101180 and 11255, drug binding, Plasma membranes, Hrh3 and IDBG-213755 and ENSMUSG00000039059 and 99296, HRH3 and IDBG-641005 and ENSBTAG00000031625 and 789851

Gene info

• Identity
  HGNC:5184
• Gene
  HRH3
• Long gene name
  histamine receptor H3
• Synonyms
  • GPCR97
• GenBank acession
  • AF140538
• Locus
  20q13.33
• Discovery year
  2000-01-07
• Entrez gene record
  11255
• Pubmed identfication
  • 10347254
• RefSeq identity
  • NM_007232
• Classification
  • Histamine receptors
• VEGA ID
  OTTHUMG00000032899

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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