His Tag Antibody
-
Catalog numberF52070-0.4ML
-
PricePlease ask
-
Size0.4 ml
-
-
CategoryAntibody
-
ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
-
FormPurified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenHis Tag
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivity; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB, ELISA
-
Recommended dilutionsWestern blot: 1:1000
-
NotesTitration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented useThis His Tag antibodyis to be used only for research purposes and not for diagnostics..
-
PurityPurified
-
DescriptionEpitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
-
ImmunogenPoly-His peptide was used as the immungen for the His Tag antibody.
-
StorageAliquot the His Tag antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Additional conjugationhistidine
-
French translationanticorps
-
Gene target
-
Short nameAnti-His Tag
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Tags
-
IsotypeRabbit Ig
-
LabelHis
-
Alternative nameAntibodies to His Tag
-
Alternative techniqueantibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data